Multiplex PCRs for identification of Escherichia coli virulence genes.

نویسندگان

  • M A Pass
  • R Odedra
  • R M Batt
چکیده

PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These reactions successfully identified genes in E. coli from the feces of four dogs.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 38 5  شماره 

صفحات  -

تاریخ انتشار 2000